Growth Inhibition by Copper and Copper Intake in Tetrahymena pyriformis GL

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The inhibitory effect of copper on the growth of Tetrahymena pyriformis GL was closely related to copper intake of the cells. The copper intake in Tetrahymena cells was different from that of yeast cells, which have a cell wall, in dependence on pH and the distribution of copper in the cells. The greater part of the copper taken in by the cells was in the fraction of pellicles and cilia, and only about 5% was in the fraction of cytoplasm. The binding of copper to cells was biphasic. Tetrahymena cells grew well in some synthetic media at a pH range of 4 to 9; the growth inhibition by copper was more clearly shown above pH 6.5. The copper intake of the whole cells decreased with increasing pH, but copper incorporation into the inside of cells increased with pH. About 50% of the copper incorporated into the cells was in the mitochondrial fraction. From these results, it is evident that growth inhibition was mainly caused by copper incorporated into the inside of cells, but the great quantity of copper bound to the cell surface at high copper concentrations might also affect cell growth.
公益社団法人日本生物工学会の論文
1984-12-25