CD36 Genotype and Long-Chain Fatty Acid Uptake in the Heart

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Homozygous or compound heterozygous mutation of the CD36 gene (CD36^<-/->) in humans results in severe defects of the uptake of long-chain fatty acids (LCFAs) in the heart. Because the effect of a single mutation of this gene (CD36^<-/->) on the LCFA uptake is not known, it was evaluated in 29 subjects with the CD36 wild-type gene (WT) (6 healthy subjects, 10 patients with heart disease), CD36^<+/-> (4 healthy subjects, 5 patients) and CD36^<-/-> (4 patients). The CD36 genotype was identified in the coding region of genomic DNA, and the expression of CD36 protein was examined by flow cytometry after staining with monoclonal anti-CD36 antibody. The LCFA uptake in the heart was assessed as the radioactivity accumulation ratio of heart to mediastinum after intravenous administration of iodine- 123 15-(p-iodophenyl)-3-R, S-methylpentadecanoic acid (H/M ratio). The HIM ratios in WT, CD36^<+/->- and CD36^<-/-> were 2.28★0.10 1.90★0.06 and 1.40★0.11 respectively (p<0.0001, among groups). The H/M ratio between healthy subjects and patients with heart disease for WT and CD36^<+/-> did not differ significantly (ie, those of WT and CD36^<+/-> in healthy subjects and patients were 2.29★0.08 vs 2.27★0.12 and 1.90★0.07 vs 1.89★0.05, respectively). Not only CD36^<-/-> but also CD36^<+/-> resulted in a significant reduction of the LCFA uptake in the heart independent of heart disease, suggesting genotype dependency and that CD36 might be a fundamental determinant of myocardial LCFA uptake.
社団法人日本循環器学会の論文
2002-08-20

CD36 Genotype and Long-Chain Fatty Acid Uptake in the Heart

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