Resting-cell Studies on Carbon-source Regulation of β-Lactam Antibiotic Biosynthesis

概要

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A resting-cell system was developed to provide information on the type of carbon regulation involved in β-lactam antibiotic biosynthesis by Cephalosporium acremonium. It consisted of washed mycelia that produced antibiotic in buffer in the absence of protein synthesis. The specific antibiotic production by the resting-cell system was considered to reflect overall "β-lactam synthetase" activity. It was found that "β-lactam synthetase"peaked early during fermentation in rapidly utilized carbon sources and later with slowly utilized carbon sources. The peak amount of "β-lactam synthetase, " which appeared during rapid glucose utilization, was large. It was thus concluded that the carbon source did not exert catabolite repression on antibiotic biosynthesis. The observation of high enzyme levels when the carbon source was present and being metabolized, coupled with the finding that antibiotic production rapidly only after glucose was depleted from the medium, suggested the involvement of catabolite inhibition of "β-lactam synthetase". Resting-cell experiments with various sugar and nitrogen additives showed that antibiotic production was inhibited only when glucose was accompanied by a nitrogen source.
社団法人日本生物工学会の論文
1978-08-25