Effects of the β-Lactamase Gene and Orientation of the Kanamycin-Resistance Gene in Plasmid pCED3 on the Growth of Bacillus subtilis

スポンサーリンク

概要

• 論文の詳細を見る

• Recombinant plasmid pCED3 inhibits growth of Bacillus subtilis cultures but in Escherichia coli, the plasmid has no effect on growth. We previously found that transcription from the tms promoter inhibits the expression of the plasmid-encoded kanamycin resistance gene (KNT) in B. subtilis resulting in the reduction of both β-galactosidase expression and cell growth rate in the presence of kanamycin. This effect was found to be markedly diminished by changign the orientation of the KNT gene on the plasmid. The primary β-lactamase gene product, which is not processed in B. subtilis, did not take part in growth inhibition. Thus the negative effect of tms-directed transcription on the cell's resistance to kanamycin and the high amount of pUB110-derived plasmid DNA in B. subtilis can account for the differential growth effect on B. subtilis and E. coli that plasmid pCED3 exerts.

• 社団法人日本生物工学会の論文
• 1991-10-25

著者

• Demain A

  Massachusetts Inst. Technol. Ma Usa

• SHOHAM YUVAL

  (Present address)Departments of Food Engineering and Biotechnology, The Technion

• STEPHANOPOULOS GREGORY

  Departments of Biology and Chemical Engineering, Massachusetts Institute of Technology

• DEMAIN ARNOLD

  Departments of Biology and Chemical Engineering, Massachusetts Institute of Technology

• Shoham Yuval

  (present Address)departments Of Food Engineering And Biotechnology The Technion

• Demain Arnold

  Department Of Biology Massachusetts Institute Of Technology

• Stephanopoulos Gregory

  Departments Of Biology And Chemical Engineering Massachusetts Institute Of Technology

関連論文

• Effects of the β-Lactamase Gene and Orientation of the Kanamycin-Resistance Gene in Plasmid pCED3 on the Growth of Bacillus subtilis
• Emerging Concepts of Secondary Metabolism in Actinomycetes
• An Unexpected Inhibitory Effect of Rapamycin against Germination of Spores fo Bacillus brevis Strain Nagano
• New Microorganisms which Convert Compactin to Pravastatin
• Role of Citrate in Xanthan Production by Xanthomonas campestris
• Resting-cell Studies on Carbon-source Regulation of β-Lactam Antibiotic Biosynthesis
• Biotransformation of Compactin to Pravastatin by Actinomadura sp. 2966

スポンサーリンク