Attachment Kinetics of Vero Cells onto CT-3 Microcarriers

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概要

• 論文の詳細を見る

• The effects of cell seeding density, microcarrier concentrations, agitation speed and age of seeding cells on the rate of cell attachment to the surface of CT-3 microcarriers were investigated. It was shown that the attachment followed first-order kinetics. When either the cell seeding density or the microcarrier concentration was increased, the kinetic constant increased due to an increase in the probability of collision between cells and microcarriers. However, at higher microcarrier concentrations, the increase in the kinetic constant with increasing microcarrier concentration was not significant. Cell attachment was declerated upon increasing the agitation speed because of the shorter cell-microcarrier contact time. In addition, it was also demonstrated that cell attachment occurred more efficiently when seeding cells from the middle or early exponential growth phase were used. The process of cell attachment ontp CT-3 microcarriers was investigated, and our results showed that the attachment stage was the rate-limiting step. These results will facilitate the optimization of the Vero cell culture process.

• 公益社団法人日本生物工学会の論文
• 2000-07-25

著者

• Zhang Yuanxing

  State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology

• SUN XIANGMING

  State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology

• TAN WENSONG

  State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology

• ZHOU YAJING

  State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology

• HUA PING

  State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology

• Hua Ping

  State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology

• Zhou Y

  Ministry Of Agriculture Sericultural Res. Inst. Chinese Acad. Agricultural Sci. Jiangsu Chn

• Tan Wensong

  State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology

• Sun Xiangming

  State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology

• Zhang Y

  East China Univ. Sci. And Technol. Shanghai Chn

• Sun Xianging

  State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology

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• Attachment Kinetics of Vero Cells onto CT-3 Microcarriers
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