Involvement of LuxS in the Regulation of Motility and Flagella Biogenesis in Vibrio alginolyticus
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概要
- 論文の詳細を見る
The fish pathogen Vibrio alginolyticus contains two unique flagellar systems. The LuxS quorum sensing system is reported to regulate the expression of virulence factors in a wide variety of pathogenic bacteria. Our previous work demonstrated that inactive luxS led to decreased virulence in V. alginolyticus. In this study, LuxS-dependent regulation of motility and flagella biogenesis, the potential virulence factors in V. alginolyticus, were further investigated. A luxS-deleted mutant showed deficiency in motility and flagella formation, and an intact luxS complemented the defect. Since motility is flagella dependent, V. alginolyticus flagella biogenesis related genes, including the flagella regulator genes flaK and lafK and the sub-hierarchical flagellar genes fliS and lafA, were cloned and identified. Based on quantitative real-time reverse transcription-PCR, differential expression of these genes was confirmed in wild-type and luxS mutants. Our results indicate that LuxS plays an important role in the regulation of motility and flagella biogenesis in V. alginolyticus.
- 社団法人 日本農芸化学会の論文
- 2008-04-23
著者
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Ma Yue
State Key Lab. Of Bioreactor Engineering East China Univ. Of Sci. And Technol.
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Liu Qin
Dep. Of Agricultural Biotechnology Coll. Of Agriculture And Life Sciences Seoul National Univ.
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Ma Yue
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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TIAN Yang
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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WANG Qiyao
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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LIU Qin
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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CAO Xiaodan
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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GUAN Lingyu
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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ZHANG Yuanxing
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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Tian Yang
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Guan Lingyu
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Wang Qiyao
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Zhang Yuanxing
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Cao Xiaodan
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
関連論文
- Involvement of LuxS in the Regulation of Motility and Flagella Biogenesis in Vibrio alginolyticus
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