Porcine Liver Esterase-Catalyzed Enantioselective Hydrolysis of a Prochiral Diester into Its Optically Pure(S)-Ester Acid, a Precursor to a Growth Hormone Secretagogue

概要

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A limited screen of several commercially-available and internally-produced lipases and esterases identified porcine liver esterase as a suitable biocatalyst for the enantioselective hydrolysis of a diester into its(S)-ester acid with high optical purity(99%). This(S)-ester acid is a precursor to an experimental growth hormone secretagogue. After identifying xanthan gum as the best emulsifier and optimizing the reaction conditions, hydrolysis rates of 1g/l・h and final(S)-ester acid(ee>99%)titers of about 8.5g/l were routinely achieved. This process supported the production of preparative amounts of optically pure(S)-ester(ee>99%)with a high reaction yield of 82%. Upon purification, the(S)-ester was successfully used in the subsequent synthetic steps to yield the growth hormone secretagogue.
公益社団法人日本生物工学会の論文
1999-03-25