Effect of Nitrogen Sources on the Levels of Aldose Reductase and Xylitol Dehydrogenase Activities in the Xylose-Fermenting Yeast Candida tenuis

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The effects of different medium components on growth of the D-xylose-fermenting yeast Candida tenuis CBS 4435 as well as on formation of aldose reductase(ALR, xylose reductase)and xylitol dehydrogenase(XDH)were investigated. By using the Plackett-Burman statistical screening method, important effects for the two N sources yeast extract and NH_4NO_3 were found, while a change in the concentration of the other medium components (D-xylose, peptone from casein, a vitamin solution, a trace element solution, KH_2PO_4)neither significantly affected growth nor enzyme synthesis in C. tenuis. The importance of these two N sources was confirmed by subsequent shaken flask experiments planned according to a full factorial design and by bioreactor cultivations. Yeast extract was identified as important for growth as welt as for the formation of ALR and XDH. Ammonium(NH_4Cl)was also shown to have a stimulalory effect on the levels of these two enzynes formed in C. tenuis, whereas it reduced growth when present in the medium in a concentratiotn of 100 mM.
社団法人日本生物工学会の論文
1998-02-25