Characterization of Elicitor-inducible Tobacco Genes Isolated by Differential Hybridization
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概要
- 論文の詳細を見る
Inducible responses in plants against pathogen attack play a major role in resistance to disease. The defense responses are mostly associated with the expression of various kinds of inducible genes. We employed differential hybridization to isolate elicitor-inducible genes (EIGs) of tobacco (Nicotiana tabacum cv. Samsun NN) using the tobacco-fungal elicitor system. A cDNA library was constructed from tobacco leaves treated for 12 hr with hyphal wall components (HWC) prepared from Phytophthora infestans, and six EIGs were identified. Expression of all EIGs was induced after inoculation with the soybean pathogen Pseudomonas syringae pv. glycinea (nonpathogenic on tobacco) or treatment with salicylic acid, and a variety of expression patterns of EIG mRNAs was observed. Sequence analysis of EIG cDNAs revealed similarities to genes for SAR8.2 (EIG-B39 and EIG-D14), glycine-rich protein (EIG-G7), extensin (EIG-I30), acyltransferase (EIG-I24) and unknown protein (EIG-J7). Possible roles of EIG products in disease resistance are discussed.(Received August 30, 2000 ; Accepted November 30, 2000)
- 日本植物病理学会の論文
- 2001-05-25
著者
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Doke Noriyuki
Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences, Nagoya University
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Takemoto Daigo
Plant Pathology Laboratory, Graudate School of Bioagricultural Sciences, Nagoya University
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Kawakita Kazuhito
Plant Pathology Laboratory, Graudate School of Bioagricultural Sciences, Nagoya University
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Takemoto Daigo
Plant Pathology Laboratory Graduate School Of Bioagricultural Sciences Nagoya University:plant Cell
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Doke Noriyuki
Plant Pathology Laboratory Graduate School Of Bioagricultural Sciences Nagoya University
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Doke Noriyuki
Plant Pathol. Lab. Grad. School Bioagr. Sci. Nagoya Univ.
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Kawakita Kazuhito
Plant Pathology Laboratory Graduate School Of Bioagricultural Sciences Nagoya University
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Kawakita Kazuhito
Graduate School Of Bioagricultural Sciences Nagoya University
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Kawakita Kazuhito
Plant Path. Lab. School Agric. Sci.nagoya Univ.
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Takemoto Daigo
Plant Pathology Laboratory Graduate School Of Bioagricultural Sciences Nagoya University
関連論文
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- Isolation of the Gene for EILP, an Elicitor-Inducible LRR Receptor-Like Protein, from Tobacco by Differential Display
- CLONING AND CHARACTERIZATION OF DISEASE STRESS-INDUCED GENES OF TOBACCO
- Molecular Cloning of a Defense-Response-Related Cytochrome P450 Gene from Tobacco
- CLONING AND CHARACTERIZATION OF CELL WALL ELICITOR-INDUCED GENES OF TOBACCO
- MOLECULAR CLONING OF CELL WALL ELICITOR-INDUCED GENES OF TOBACCO BY DIFFERENTIAL DISPLAY
- EXPRESSION OF VACUOLAR PROCESSING ENZYME GENE(S) IN TOBACCO LEAVES TREATED WITH ELICITORS
- Characterization of Elicitor-inducible Tobacco Genes Isolated by Differential Hybridization
- ISOLATION OF ELICITOR-RESPONSIVE GENES IN POTATO BY SUBTRACTION METHOD
- THE EXPRESSION OF 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID SYNTHASE GENES IN POTATO LEAVES INFECTED WITH Phytophthora infestans OR TREATED WITH ELICITORS
- Identification of Chitinase and Osmotin-Like Protein as Actin-Binding Proteins in Suspension-Cultured Potato Cells
- INVOLVEMENT OF ACTIN AND ACTIN-RELATED PROTEIN(S)IN ELICITATION OF DEFENCE RESPONSE IN POTATO PLANT
- Induction of 3-Hydroxy-3-methylglutaryl CoA Reductase in Potato Tubers after Slicing, Fungal Infection or Chemical Treatment, and Some Properties of the Enzyme
- Expression of Genes for Phenylalanine Ammonia-Lyase and 3-Hydroxy-3-Methylglutaryl CoA Reductase in Aged Potato Tubers Infected with Phytophthora infestans
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