Development of Increased Oxolinic Acid-resistance in Burkholderia glumae
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概要
- 論文の詳細を見る
Genomic DNA, partially digested with Sau3AI, of Burkholderia glumae Pg-13, resistant to oxolinic acid (OA), was ligated into pUC118, and Escherichia coli DH5α resistant to 2.5 μg/ml OA was transformed with the plasmid. After incubation on a medium supplemented with 20 μg/ml OA, a clone harboring pB´46 was selected. The nucleotide sequence of the 724-bp insert of pB´46 had no homology to any characterized gene. B. glumae Pg-5, resistant to 10 μg/ml OA, was transformed with plasmid pUCD3101B´46 containing the insert. An obtained transformant, B25, was resistant to 50 μg/ml OA and had greater resistance to other quinolones than did Pg-5. Transformants of OA-sensitive B. glumae with pUCD3101B´46 had OA sensitivity similar to that of the parental isolates.
- 日本植物病理学会の論文
- 2001-02-25
著者
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Okuno Tetsuro
Laboratory Of Plant Pathology Graduate School Of Agriculture Kyoto University
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HIKICHI Yasufumi
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
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Hikichi Yasufumi
Laboratory Of Plant Pathology & Biotechnology Faculty Of Agriculture Kochi University
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MAEDA Yukiko
Laboratory of Plant Pathology and Biotechnology, Kochi University
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Maeda Yukiko
Laboratory Of Plant Pathology & Biotechnology Faculty Of Agriculture Kochi University
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Okuno Tetsuro
Laboratory Of Plant Pathology & Biotechnology Faculty Of Agriculture Kochi University:laboratory
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TSUJIGUCHI Kyoko
Laboratory of Plant Pathology & Biotechnology, Faculty of Agriculture, Kochi University
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Tsujiguchi Kyoko
Laboratory Of Plant Pathology & Biotechnology Faculty Of Agriculture Kochi University
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