Detection of Tobamoviruses from Soils by Non-precoated Indirect ELISA

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概要

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• A method for detecting tobamoviruses from field soils was developed using non-precoated indirect enzyme-linked immunosorbent assay(Id-ELISA). Absorbance values in Id-ELISA were relatively low after directly applying Pepper mild mottle virus(PMMoV)-infested soil extract. However, heat treating the soil extract before application greatly enhanced the absorbance values. The heat treatment was essential for the Id-ELISA detection of tobamoviruses from infested soil, although the efficiency of virus recovery varied depending on the properties of soil. The number of local lesions in the infectivity assay was consistent with the absorbance values in Id-ELISA. Moreover, the absorbance values in Id-ELISA were correlated with the incidence of soil transmission of PMMoV. Thus, Id-ELISA combined with heat treatment is a practical technique for the diagnosis of infestation with Tobamovirus in field soils, Gray Lowland soil and Sand-dune Regosol.

• 日本植物病理学会の論文
• 2000-05-25

著者

• Okuno T

  Kochi Univ. Kochi Jpn

• Okuno Tetsuro

  Laboratory Of Plant Pathology Graduate School Of Agriculture Kyoto University

• TAKEUCHI Shigeharu

  Laboratory of Plant Pathology, Kochi Agricultural Research Center

• HIKICHI Yasufumi

  Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University

• KAWADA Yoichi

  Laboratory of Plant Pathology, Kochi Agricultural Research Center

• Kawada Yoichi

  Laboratory Of Plant Pathology Kochi Agricultural Research Center

• Hikichi Y

  Laboratory Of Plant Pathology And Biotechnology Kochi University

• Hikichi Yasufumi

  Laboratory Of Plant Pathology & Biotechnology Faculty Of Agriculture Kochi University

• Okuno Tetsuro

  Laboratory Of Plant Pathology & Biotechnology Faculty Of Agriculture Kochi University:laboratory

• Takeuchi S

  Kochi Agricultural Res. Center Kochi Jpn

• Hikichi Yasufumi

  Laboratory Of Plant Pathology And Biotechnology Kochi University

関連論文

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