Asymmetric Bioreduction of a Keto Ester to Its Corresponding (S)-Hydroxy Ester by Microbacterium sp. MB 5614

スポンサーリンク

概要

• 論文の詳細を見る

• A bioprocess employing Microbacterium sp. MA 5614 was developed for the productin of the MK-0476 (S)-hydroxy ester by asymmetric bioreduction of the corresponding keto ester. The cystinyl leukotriene receptor antagonist MK-0476 [3-[(1S)-[3(E)-[2-(7-chloroquinolinyl)ethenyl]phenyl]-3-(acetylphenyl)-propylthiol]-2(S)-methylpropanoic acid] is currently undergoing clinical evaluation for the treatment of asthma. Optimization of the bioconversion medium composition and the time of keto ester addition, as well as the development of a keto ester feeding strategy yielded improvements in the bioreduction rate and final (S)-hydroxy ester titer, by 20- and 25-fold respectively. This asymmetric bioreduction process supported the production of preparative quantities of (S)-hydroxy ester with an enantiomeric excess greater than 95%.

• 社団法人日本生物工学会の論文
• 1996-06-25

著者

• Chartrain M

  Merck Res. Lab. Nj Usa

• Roberge C

  Departments Of Bioprocess Research And Development:merc Research Laboratories

• Roberge Christopher

  Merck Research Laboratories

• Greasham R

  Merck Res. Lab. Nj Usa

• GREASHAM RANDOLPH

  Merck Research Laboratories

• KING ANTHONY

  Merck Research Laboratories

• PECORE VICTOR

  Merck Research Laboratories

• CHARTRAIN MICHEL

  Merch Research Laboratories

• Pecore V

  Merck Res. Lab. New Jersey Usa

• King Anthony

  Process Research Merck Research Laboratories

関連論文

• Enhancement of Lipase Production during Fed-Batch Cultivation of Pseudomonas aeruginosa MB 5001
• Production of cis-1, 2-Dihydroxy-3-Methylcyclohexa-3, 5-Diene (Toluene cis Glycol) by Rhodococcus sp. MA 7249
• Bioconversion of Indene to cis(1S, 2R)Indandiol and trans(1R, 2R)Indandiol by Rhodococcus Species
• Chiral Bio-Resolution of Racemic Indene Oxide by Fungal Epoxide Hydrolases
• Process Development for the Producton of the (S)-Acid Precursor of a Novel Elastase Inhibitor (L-694,458) through the Lipase-Catalyzed Kinetic Resolution of a β-Lactam Benzyl Ester
• Asymmetric Bioreduction of a Keto Ester to Its Corresponding (S)-Hydroxy Ester by Microbacterium sp. MB 5614
• Asymmetric Bioreduction of a β-Tetralone to Its Corresponding (S)-Alcohol by the Yeast Trichosporon capitatum NY 1890
• Implementation of a Rapid Microbial Screening Procedure for Biotransformation Activities
• Asymmetric Direduction of 1, 2-Indanedione to cis (1S, 2R) Indanediol by Trichosporon cutaneum MY 1506
• Asymmetric Bioreduction of Benzyl Acetoacetate to Its Corresponding Alcohol, Benzyl (S)-(+)-3-Hydroxybutyrate by the Yeast Candida schatavii MY 1831

スポンサーリンク