Effects of Volatile Fatty Acids on Methanogenesis of Methanol and of Pregrowth with Methanol on Acetate Utilization by Methanogens
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概要
- 論文の詳細を見る
The effect of acetic acid and/or propinonic acid on thermophilic methanogenesis of methanol as a sole substrate was studied using Methanosarcina species (MST-A1,CHTI-55 and MP), M. thermophila(TM-1)and two isolated strains from a thermophilic digester. All strains were resistant up to approximately 10 g/l of volatile fatty acid in a methanol medium containing yeast extract and peptone. Deletion of yeast extract and peptone from the medium, however, reduced resistivity to propionic acid by half but did not reduce it to acetic acid. Methanogenesis is affected more by pH in the presence of propionic acid than in the presence of acetic acid. Methanosarcina sp. (CHTI-55) grown on methanol could grow on acetate as the sole substrate when inoculated at the logarithmic growth phase or stationary phase within a few days after exhaustion of methanol. However, when methanol grown cultures incubated for more than 1 week after exhaustion of methanol were inoculated to fresh methanol or acetate medium, the methanogen was able to grow with methanol but not with acetate. When methanogens which had lost their acetate-utilizing ability were inoculated to methanol-containing medium, the acetate-utilizing ability was recovered.
- 社団法人日本生物工学会の論文
- 1989-12-25
著者
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Tanimoto Youichi
Institute Of Technology Shimizu Construction Co. Ltd.
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Yamaguchi Makoto
Advanced Technology Research Department Institute Of Technology Shimizu Corporation
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Okamura K
Advanced Technology Research Department Institute Of Technology Shimizu Corporation
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TANIMOTO YUICHI
Advanced Technology Research Department, Institute of Technology, Shimizu Corporation
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OKAMURA KAZUO
Advanced Technology Research Department, Institute of Technology, Shimizu Corporation
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MINAMI KIYOSHI
Advanced Technology Research Department, Institute of Technology, Shimizu Corporation
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Okamura Kazuo
Advanced Technology Research Department Institute Of Technology Shimizu Corporation
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Okamura K
Mercian Corp. Kanagawa Jpn
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Minami K
Shimizu Corp. Tokyo Jpn
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- Effects of Volatile Fatty Acids on Methanogenesis of Methanol and of Pregrowth with Methanol on Acetate Utilization by Methanogens
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