Possible Use of a 1.8kb DNA Fragment for the Specific Detection of Salmonella in Foods

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概要

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• The aim of this study was to find a specific DNA probe for the detection of Salmonella in foods. A 1.8kb HindIII DNA fragment cloned from S. typhimurium was tested for its specificity by hydridization to Salmonella of different serotypes as well as to non-Salmonella bacteria. The ^<35> S-labeled HindIII DNA fragment hybridized with all of the common and uncommon Salmonella isolates but did not hybridize with the other bacteria tested. Hybridization efficiencies of this 1.8kb DNA probe to Salmonella strains of different serotypes were nearly the same. When this probe was applied to the detection of Salmonella exogenously added to a variety of foods, bacterial flora naturally occurring in foods did not interfere with the hybridization assay. Thus, the hybridization specificity of this 1.8kb DNA probe was established.

• 公益社団法人日本生物工学会の論文
• 1989-07-25

著者

• Wang S‐j

  East China Univ. Sci. And Technol. Shanghai Chn

• Tsen Hau-yang

  Department Of Food Science National Chung Hsing University

• CHEN MEI-HUA

  Department of Food Science, National Chung Hsing University

• SHIEH JIUNN-SHIUH

  Department of Food Science, National Chung Hsing University

• WANG SHU-JEN

  Department of Food Science, National Chung Hsing University

• HU NIEN-TAI

  Department of Food Science, National Chung Hsing University

• Hu Nien-tai

  Department Of Food Science National Chung Hsing University

• Chen Mei-hua

  Department Of Food Science National Chung Hsing University

• Shieh Jiunn-shiuh

  Department Of Food Science National Chung Hsing University

関連論文

• Comparison of the Kinetics and Factors Affecting the Stabilities of Chitin-Immobilized Naringinases from Two Fungal Sources
• Possible Use of a 1.8kb DNA Fragment for the Specific Detection of Salmonella in Foods
• Possible Use of a Polymerase Chain Reaction Method for Specific Detection of Salmonella in Beef
• Fiber Entrapment of Naringinase from Penicillium sp. and Application to Fruit Juice Debittering
• Factors Affecting the Inactivation of Naringinase Immobilized on Chitin during Debittering of Fruit Juice
• A Colorimetric Microtiter Plate Sandwich DNA Hybridization System for the Detection of Salmonella in Foods
• Isolation and evaluation of lactic acid bacteria with probiotic and immunopotentiating functions

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