Assay of DNA Photolyase Activity in Spinach Leaves in Relation to Cell Compartmentation-Evidence for Lack of DNA Photolyase in Chloroplasts
スポンサーリンク
概要
- 論文の詳細を見る
Spinach cyclobutane pyrimidine dimer (CPD)-specific DNA photolyase was successfully detected in leaf extracts by an assay system for plant photolyase using an improved enzyme-linked immunosorbent assay (ELISA) which was newly introduced by novel horseradish peroxidase (HRP)-linked CDP specific monoclonal antibodies. The assay system includes two main steps : a photorepair reaction of CPD introduced in substrate DNA and measurement of CPD remained after the photorepair by the improved ELISA. When CPD-induced salmon sperm DNA was used as a substrate, high CPD-photolyase activities were observed in the enzyme fraction prepared from whole spinach leaf extracts, but not from chloroplast extracts. This strongly suggests that spinach CPD-specific photolyases are localized in cell compartments other than chloroplasts.
- 社団法人日本農芸化学会の論文
- 2000-06-23
著者
-
HINO Keisuke
Yamaguchi University School of Medicine
-
HINO KEISUKE
Department of Gastroenterology and Hepatology, Yamaguchi University School of Medicine
-
Goss Jorn
Institute Of Biology Ii Freiburg University
-
HADA Megumi
Department of Biology, Faculty of Science, Kobe University
-
BUCHHOLZ Gunther
Institute of Biology II, Freiburg University
-
WELLMANN Eckard
Institute of Biology II, Freiburg University
-
SHIN Masateru
Teru Biolaboratory
-
Hino K
First Department Of Internal Medicine Yamaguchi University School Of Medicine Saiseikai Shimonoseki
-
Hino Keisuke
First Department Of Internal Medicine Yamaguchi University School Of Medicine
-
Hino Keisuke
Department Of Biology Faculty Of Science Kohnan Universtiy:(present Address) Institute Of Advanced E
-
Hino Keisuke
Department Of Basic Laboratory Sciences Yamaguchi University Graduate School Of Medicine
-
Hino Keisuke
Department Of Gastroenterology And Hepatology Yamaguchi University School Of Medicine
-
Buchholz Gunther
Institute Of Biology Ii Freiburg University
-
Hada M
Department Of Biology Faculty Of Science Kobe University
-
Hada Megumi
Department Of Biology Faculty Of Science Kobe University:(present Address)department Of Bioscience A
-
Hada Megumi
Biology Department Brookhaven National Laboratory
-
Hino Keisuke
Department Of Laboratory Sciences Yamaguchi University School Of Medicine
-
Wellmann Eckard
Institute Of Biology Ii Freiburg University
関連論文
- Spatial and chronological differences in hepatitis B virus genotypes from patients with acute hepatitis B in Japan
- Interferon therapy for aged patients with chronic hepatitis C : improved survival in patients exhibiting a biochemical response
- Assay of DNA Photolyase Activity in Spinach Leaves in Relation to Cell Compartmentation-Evidence for Lack of DNA Photolyase in Chloroplasts
- LIGHT REGURATION AND ACTION SPECTRA OF PHOTOLYASES FOR CYCLOBUTANE PYRIMIDINE DIMER (CPD) AND (6-4) PHOTOPRODUCT IN BROOM SORGHUM SEEDLINGS
- Hepatitis C virus core protein inhibits deoxycholic acid-mediated apoptosis despite generating mitochondrial reactive oxygen species
- In situ detection of oxidized n-3 polyunsaturated fatty acids in chronic hepatitis C : correlation with hepatic steatosis
- Hepatitis C virus infection upregulates expression of the type I interferon receptor in human peripheral blood mononuclear cells
- Hepatic expression of type I interferon receptor for predicting response to interferon therapy in chronic hepatitis C patients : a comparison of immunohistochemical method vs. competitive polymerase chain reaction assay
- Hepatitis viral infection in Behcet's disease
- PHOTOREPAIR OF PYRIMIDINE DIMERS AND PHOTOLYASE INDUCTION IN BROOM SORGHUM SEEDLINGS
- PHOTOREPAIR OF PYRIMIDINE DIMERS AND PHOTOLYASE INDUCTION IN BROOM SORGHUM SEEDLINGS
- Detection of hepatitis B virus DNA in liver by polymerase chain reaction for the diagnosis of fulminant hepatitis B
- One-point quantitative determination of pretreatment serum hepatitis C virus RNA predicts long-term responsiveness to high-dose interferon therapy
- Intrahepatic hepatitis C virus RNA levels after interferon treatment: eradication of the minus-strand RNA correlates with sustained remission of hepatitis
- Hepatitis B virus genotype G is an extremely rare genotype in Japan
- Therapeutic effects of stronger neo-minophagen C at different doses on chronic hepatitis and liver cirrhosis
- Efficacy of interferon alfacon-1 daily induction therapy followed by three-times weekly therapy in patients with chronic hepatitis C virus infection with high and low viral titers
- Involvement of GBV-C/HGV in liver diseases in Japan
- New Inuyama classification; new criteria for histological assessment of chronic hepatitis^1
- Clinical evaluation of chronic liver diseases induced by different hepatitis C virus genotype in Japan, focused on the difference between genotypes III/2a and IV/2b
- Changes of depolymerized and polymerized tubulin proteins in the liver after taurocholic acid administration with or without colchicine pretreatment and cholestasis due to cytochalasin B and bile duct ligation in rats
- Hepatocellular carcinoma developing six and a half years after a diagnosis of idiopathic portal hypertension
- Management of hepatitis C ; Report of the Consensus Meeting at the 45th Annual Meeting of the Japan Society of Hepatology (2009)
- Immunohistochemical study study of Fas antigen in liver of Patients with chronic hepatitis and autoimmune liver disease
- A Case Report of Seuretin Therapy for Primary Sclerosing Cholangitis
- PURIFICATION OF MULBERRY (Mlorus bombycis Koidz) FERREDOXIN AND DETERMINATION OF ITS PRIMARY STRUCTURE
- A Case of Ovulatory Cycle-dependent Symptoms in Woman with Previous Interferon β Therapy
- Hepatitis B Virus Carrier Status Linked to Autoimmune Hepatitis
- "Rebound" phenomenon of hepatitis C viremia after interferon therapy in "relapsed" patients with chronic hepatitis C
- Clustered DNA Damages Induced in Human Hematopoietic Cells by Low Doses of Ionizing Radiation
- Focal Nodular Hyperplasia-Like Nodule with Reduced Expression of Organic Anion Transporter 1B3 in Alcoholic Liver Cirrhosis
- Action Spectra of DNA Photolyases for Photorepair of Cyclobutane Pyrimidine Dimers in Sorghum and Cucumber
- Development of UV Defense Mechanisms during Growth of Spinach Seedlings
- Recommendation of lamivudine-to-entecavir switching treatment in chronic hepatitis B responders : Randomized controlled trial