Enzyme-Linked Immunosorbent Assay for Detection of Chitooligosaccharides

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概要

• 論文の詳細を見る

• In order to detect chitooligosaccharides (COS), an enzyme-linked immunosorbent assay (ELISA) was developed. A chitooligosaccharide mixture (COSM) conjugated to bovine serum albumin was used to immunize rabbits to produce an anti-COS polyclonal antibody. By use of specific antibody and COSM-horseradish peroxidase conjugate, we established a competitive direct ELISA (cdELISA) the detection limit of which was about 0.1 μg/ml. In the cdELISA, the cross-reactivities of the specific antibody toward glucosamine, chitobiose, chitotriose, chitotetraose, chitopentaose, and chitohexaose were 0.27,27,75,75,144,and 100%, respectively, and those toward N-acetylchitobiose, N-acetylchitotriose, N-acetylchitotetraose, N-acetylchitopentaose, and N-acetylchitohexaose were 1.58,0.005,1.08,0.05,and 0.40%, respectively.

• 社団法人日本農芸化学会の論文
• 2000-04-23

著者

• Shon Dong-hwa

  Food Chemistry And Biotechnology Division Korea Food Research Institute

• LEE Ke-ho

  Department of Food Science & Technology, Colege of Agriculture & Life Science, Seoul National Univer

• Lee Ke-ho

  Department Of Food Science And Technology College Of Agricultural Life Sciences Seoul National Unive

• KIM SOON-YOUNG

  Department of Food Science and Technology, College of Agricultural Life Sciences, Seoul National Uni

• Kim Soon-young

  Department Of Food Science And Technology College Of Agricultural Life Sciences Seoul National Unive

関連論文

• Benzastatins A, B, C, and D: New Free Radical Scavengers from Streptomyces nitrosporeus 30643 I. Taxonomy, Fermentation, Isolation, Physico-chemical Properties and Biological Activities
• Enzyme-Linked Immunosorbent Assay for Detection of Chitooligosaccharides

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