Role of the Non-essential Region Encompassing the N-Terminal Two Transmembrane Stretches of Escherichia coli SecE
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概要
- 論文の詳細を見る
SecE is an essential component of the protein translocation machinery of Escherichia coli and has three transmembrane stretches. An N-terminal region (SecE-N) encompassing the first two transmembrane stretches is dispensable for protein translocation but a SecE derivative (SecE-C) lacking this region is very unstable. We show here that FtsH, the AAA (ATPases associated with diverse cellular activities) family protease, causes the instability of SecE-C. SecE-C became stable when SecE-N was co-expressed. Deletion of the N-terminal region of SecE also rendered the SecE-SecY-SecG complex unstable. In spite of these alterations, the N-terminal region of SecE had little stimulatory effect on protein translocation in vivo or in vitro.
- 社団法人日本農芸化学会の論文
- 2000-10-23
著者
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Tokuda Hajime
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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NISHIYAMA Ken-ichi
Institute of Molecular and Cellular Biosciences, The University of Tokyo
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NISHIYAMA Kenichi
Institute of Molecular and Cellular Biosciences, The University of Tokyo
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SUZUKI Hirofumi
Institute of Molecular and Cellular Biosciences, The University of Tokyo
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Nishiyama Ken-ichi
Institute Of Geoscience University Of Tsukuba
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Nishiyama Kenichi
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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Suzuki Hirofumi
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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Tokuda Hajime
Institute at Molecular and Cellular Bioscience. The University of Tokyo
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- Role of the Non-essential Region Encompassing the N-Terminal Two Transmembrane Stretches of Escherichia coli SecE
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