Membrane Topology and Functional Importance of the Periplasmic Region of ABC Transporter LolCDE

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概要

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• The LolCDE complex is an ATP-binding cassette transporter that mediates the release of newly synthesized lipoproteins from the cytoplasmic membrane of gram-negative bacteria, which results in the initiation of outer-membrane sorting of lipoproteins through the Lol pathway. LolCDE is composed of one copy each of membrane subunits LolC and LolE, and two copies of nucleotide-binding subunit LolD. In this study, we examined the membrane topology of LolC and LolE by PhoA fusion analysis. Both LolC and LolE were found to have four transmembrane segments with a large periplasmic loop exposed to the periplasm. Despite similarities in sequence and topology, the accessibility of a sulfhydryl reagent to Cys introduced into the periplasmic loop suggested that the structure of the periplasmic region differs between LolC and LolE. Inhibition of the release of lipoproteins by the sulfhydryl reagent supported a previous proposal that LolC and LolE have distinct functions.

• 社団法人 日本農芸化学会の論文

著者

• Tokuda Hajime

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• NARITA Shin-ichiro

  Institute of Molecular and Cellular Bioscience, The University of Tokyo

• MATSUYAMA Shin-ichi

  Department of Life Science, Rikkyo University

• Yasuda Masaki

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• IGUCHI-YOKOYAMA Asako

  Institute of Molecular and Cellular Biosciences, The University of Tokyo

• Matsuyama Shin-ichi

  Department Of Life Science Rikkyo University

関連論文

• Membrane Topology Inversion of SecG Detected by Labeling with a Membrane-Impermeable Sulfhydryl Reagent that Causes a Close Association of SecG with SecA
• Two SecG Molecules Present in a Single Protein Translocation Machinery Are Functional Even after Crosslinking
• Role of the Non-essential Region Encompassing the N-Terminal Two Transmembrane Stretches of Escherichia coli SecE
• A New Screening Method to Identify Inhibitors of the Lol (Localization of lipoproteins) System, a Novel Antibacterial Target
• Disruption of rpmJ Encoding Ribosomal Protein L36 Decreases the Expression of secY Upstream of the spc Operon and Inhibits Protein Translocation in Escherichia coli
• Biogenesis of Outer Membranes in Gram-Negative Bacteria
• Roles of the Conserved Cytoplasmic Region and No-Conserved Carboxy-Terminal Region of SecE in Escherichia coli Protein Translocase
• Photo-Hall Effect of Zinc Sulfide Crystals
• Membrane Topology and Functional Importance of the Periplasmic Region of ABC Transporter LolCDE
• Membrane Topology and Functional Importance of the Periplasmic Region of ABC Transporter LolCDE
• Biogenesis of Outer Membranes in Gram-Negative Bacteria

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