Role of Carbohydrate Moiety in Carboxypeptidase Y : Structural Study of Mutant Enzyme Lacking Carbohydrate Moiety

概要

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To study the roles of the carbohydrate moiety in the function of carboxypeptidase Y, asparagine residues at 13,87,168,and 368,the four-consensus N-linked glycosylation sites, were altered to alanine with site-directed mutagenesis. The mutant enzyme of 51 kDa completely lost the carbohydrate moiety which was present in the 61-kDa wildtype enzyme. Structural studies of the mutant enzyme showed that it maintained the native-like structure ; hydrolytic activity, and substrate specificity of the mutant enzyme analogous to those of the wild-type enzyme. Susceptibility of the mutant enzyme toward proteolysis and pressure denaturation was reduced by 10-20%. It is concluded that the carbohydrate moiety functions to maintain the structural integrity of the enzyme under stressed.
社団法人日本農芸化学会の論文
1999-06-23