An Isoelectric Separation of Soybean β-Amylase Isoforms and Their Enzymic Characteristics

スポンサーリンク

概要

• 論文の詳細を見る

• Authentic soybean β-amylase preparation, purified to homogeneity as judged by SDS-PAGE by using an affinity purification step, was composed of four pI-differing isoforms. By chromatofocusing, these isoforms were separated into three fractions, designated as fractions 1-3 in the order of elution. Fraction 1 contained two isoforms having the same molecular mass (55,989 Da), as measured by mass spectrometric analysis, with different pIs, 5.32 (Isoform I) and 5.22 (Isoform II). Fraction 2 showed a single isoform having a molecular mass of 55,994 Da and having a pI of 5.09. This component, named Isoform III, existed rather in excess in a mixture of the authentic enzyme isoforms. The remainder (fraction 3) also contained a single component (Isoform IV) which has a molecular mass of 56,310 Da with a pI of 4.97. Chemical analyses indicated that the Ntermini and the C-terminal tripeptides of four pI-separated isoforms mentioned are similar to one another, and are blocked and are NH_2-Val-Asp-Gly-COOH, respectively. Moreover, enzymic properties involving specific activity and the value of k_<cat>/K_m for the above three fractions are almost the same, and also agreed completely with those of an unfractionated authentic β-amylase preparation.

• 社団法人日本農芸化学会の論文
• 1999-04-23

著者

• Arahira Masaomi

  Genetic Engineering Laboratory National Food Research Institute Ministry Of Agriculture Forestry And

• Fukazawa Chikafusa

  Genetic Engineering Laboratory Division Of Applied Microbiology National Food Research Institute Min

• Fukazawa Chikafusa

  Genetic Engineering Laboratory National Food Research Institute Ministry Of Agriculture Forestry And

• WANG Yuhuan

  Genetic Engineering Laboratory, National Food Research Institute, Ministry of Agriculture, Forestry

• Wang Yuhuan

  Genetic Engineering Laboratory National Food Research Institute Ministry Of Agriculture Forestry And

関連論文

• Molecular Cloning and Characterization of a Group II Chaperonin σ-Subunit from Soybean
• Molecular Cloning and Expression Patterns of Cu/Zn-Superoxide Dismutases in Developing Soybean Seeds
• Molecular Cloning and Ethylene-Inducible Expression fo Chib1 Chitinase from Soybean (Glycine max (L.) Merr.)
• Molecular Cloning of the xylA Gene Encoding Xylose Isomerase from Streptomyces griseofuscus S-41 : Primary Structure of the Gene and Its Product(Biological Chemistry)
• Molecular Cloning of Mouse Peptidylarginine Deiminase, and Its Possible Isozyme cDNAs(Biological Chemistry)
• An Isoelectric Separation of Soybean β-Amylase Isoforms and Their Enzymic Characteristics

スポンサーリンク