Molecular Cloning and Ethylene-Inducible Expression fo Chib1 Chitinase from Soybean (Glycine max (L.) Merr.)

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概要

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• A soybean seed-specific PR-8 chitinase, named Chib2,has a markedly extended C-termenal segment compared to other plant Chib1 homologues of the PR-8 chitinase family known to date. To further characterize the molecular structure and the expression pattern of this chitinase family, we cloned two typical Chib1-similar cDNAs (Chib1-1 and Chib1-2) from soybeans by PCR-cloning techniques. The deduced primary sequenece of Chib1-1 chitinase is composed of a signal peptide segment (26 amino acid residues) and a mature 273 amino acid sequence (calculated molecular mass 28,794,calculated pI 7. 3). This Chib1-1 enzyme is more than 90% Chib2 enzyme. Thus, we confirmed the occurrence of two distinct classes, Chib1 and Chib2 in the plant PR-8 chitinase family. The Chib1 genes, interrupted by one intron, were found to be up-regulated in response to ethylene in stems and leaves, but scarcely expressed in developing soybean seeds. Chib1 chitinases may be responsble for protecting the plant body from various pathogenic attacks.

• 社団法人日本農芸化学会の論文
• 1999-02-23

著者

• ZHANG Deyu

  Genetic Engineering Laboratory, National Food Research Institute, Ministry of Agriculture, Forestry

• Arahira M

  Genetic Engineering Laboratory National Food Research Institute Ministry Of Agriculture Forestry And

• Arahira Masaomi

  Genetic Engineering Laboratory National Food Research Institute Ministry Of Agriculture Forestry And

• Nong Van

  Genetic Engineering Laboratory National Food Research Institute Ministry Of Agriculture Forestry And

• Udaka Kyoko

  Genetic Engineering Laboratory National Food Research Institute Ministry Of Agriculture Forestry And

• Zhang D

  Genetic Engineering Laboratory National Food Research Institute Ministry Of Agriculture Forestry And

• Fukazawa C

  Genetic Engineering Laboratory National Food Research Institute Ministry Of Agriculture Forestry And

• Fukazawa Chikafusa

  Genetic Engineering Laboratory Division Of Applied Microbiology National Food Research Institute Min

• WATANABE Akira

  Genetic Engineering Laboratory, National Food Research Institute

• YEBOAH Nana

  Genetic Engineering Laboratory, National Food Research Institute

• UDAKA Kyoko

  Tokyo Kasei University

• Yeboah Nana

  Genetic Engineering Laboratory National Food Research Institute

• Watanabe Akira

  Genetic Engineering Laboratory National Food Research Institute

関連論文

• Molecular Cloning and Characterization of a Group II Chaperonin σ-Subunit from Soybean
• Molecular Cloning and Expression Patterns of Cu/Zn-Superoxide Dismutases in Developing Soybean Seeds
• Molecular Cloning and Ethylene-Inducible Expression fo Chib1 Chitinase from Soybean (Glycine max (L.) Merr.)
• Molecular Cloning of the xylA Gene Encoding Xylose Isomerase from Streptomyces griseofuscus S-41 : Primary Structure of the Gene and Its Product(Biological Chemistry)
• Molecular Cloning of Mouse Peptidylarginine Deiminase, and Its Possible Isozyme cDNAs(Biological Chemistry)
• An Isoelectric Separation of Soybean β-Amylase Isoforms and Their Enzymic Characteristics

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