The Expression of proUK in Escherichia coli : The vgb Promoter Replaces IPTG and Coexpression of argU Compensates for Rare Codons in a Hypoxic Induction Model
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概要
- 論文の詳細を見る
The expression of the proUK gene was improved by the coexpression of the argU gene cloned in a moderate copy number vector. As the proUK gene contains 2% AGG/AGA condons, which is much higher than the normal frequency in E. coli, about 0.14%-0.21%, the argU gene cloned in a multicopy plasmid was coexpressed with the proUK expression vector in our experiments. In E. coli strain BL21(DE3), IPTG is known to induce the expression of T7 RNA polymerase gene and this enzyme can transcribe the proUK gene under the control of the T7 promoter leading to expression of proUK. To replace IPT G by a cheaper alternative on a large scale, we constructed a plasmid in which the vgb promoter-which is known to be activated by the onset of hypoxic conditions-controls the T7RNA polymerase gene expression. Low oxygen conditions were then used to activate the vgb promoter causing T7RNA polymerase gene expression and finally leading to the expression of proUK as inactive inclusion bodies. Our experiments on a large scale in a bioreactar show that the expression of proUK accounts for about 30% of total protein after about 6 h of anaerobic cultivation, so the presented model represents an economical alternative to IPTG induction.
- 社団法人日本農芸化学会の論文
- 1999-12-23
著者
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Jiang Lan
Shanghai Research Center Of Biotechnology Chinese Academy Of Sciences:institute For Medical Neurobio
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YANG SHENGLI
Shanghai Research Center of Biotechnology, Academic Sinica
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Yang S
Shanghai Res. Center Of Biotechnol. Acad. Sinica Shanghai Chn
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Yang Shengli
Shanghai Research Center Of Biotechnology
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YANG Yonghua
Shanghai Research Center of Biotechnology, Chinese Academy of Sciences
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CHATTERJEE Shampa
Institute for Medical Neurobiology, Otto-von-Guericke University
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SEIDEI Bertolt
Institute for Medical Neurobiology, Otto-von-Guericke University
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WOLF Gerald
Institute for Medical Neurobiology, Otto-von-Guericke University
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Wolf Gerald
Institute For Medical Neurobiology Otto-von-guericke University
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Yang Yonghua
Shanghai Research Center Of Biotechnology Chinese Academy Of Sciences
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Seidei Bertolt
Institute For Medical Neurobiology Otto-von-guericke University
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Chatterjee Shampa
Institute For Medical Neurobiology Otto-von-guericke University
関連論文
- Construction and Selection of the Novel Recombinant Escherichia coli Strain for Poly(β-Hydroxybutyrate) Production
- The Expression of proUK in Escherichia coli : The vgb Promoter Replaces IPTG and Coexpression of argU Compensates for Rare Codons in a Hypoxic Induction Model
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