Construction and Selection of the Novel Recombinant Escherichia coli Strain for Poly(β-Hydroxybutyrate) Production

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Heterogeneous cloning of Vitreoscilla hemoglobin gene (vgb), lytic genes of phage λ with S amber mutation (S^-RRz) and PHB biosynthetic genes (phbCAB) in the same host strain E.coli JM105 was carried out for production of poly(β-hydroxybutyrate)(PHB). A superior novel strain, VG1(pTU14), was constructed and selected, which contained the vgb gene in the chromosomal DNA and the plasmid pTU14 containing S^-RRz and phbCAB genes. When cultured in 100 ml of LBG medium in a 300-ml flask, all of the exogenous genes in VG1 (pTU14) were expressed. The cell concentration of VG1 (pTU14) grown by batch culture in a flask reached 10.2 gl^<-1> ; PHB concentration, PHB content and PHB yield, which is the ratio of the PHB accumulation to the glucose consumption, were 8.54 gl^<-1> 84% and 0.43 gg^<-1> respectively. When cultured by batch-feeding of glucose in a 300-ml shaking flask, the cell concentration and PHB content reached 26 gl^<-1> and over 96%, respectively.
社団法人日本生物工学会の論文
2000-04-25