Use of Escherichia coli Polyphosphate Kinase for Oligosaccharide Synthesis

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概要

• 論文の詳細を見る

• The Escherichia coli polyphosphate kinase (PPK) has been known to catalyze the reversible transfer of phosphate molecules between ATP and polyphosphate (poly (P)). It has also been found that the PPK catalyzes the kination of not only ADP but also other nucleoside diphosphates (NDPs) using poly (P) as a phosphate donor, yielding nucleotide triphosphates (NTPs). We used the PPK and poly (P) in place of pyruvate kinase and phosphoenol pyruvate for NTP regeneration followed by synthesis of sugar nucleotides in a cyclic synthesis system for oligosaccharides. It was confirmed that the PPK efficiently catalyzed the UTP regeneration in the cyclic system of N-acetyllac-tosamine synthesis. This novel activity of PPK enables us to perform the practical synthesis of oligosaccharides.

• 社団法人日本農芸化学会の論文
• 1998-08-23

著者

• NOGUCHI TOSHITADA

  Biochemicals Division, Yamasa Corporation

• Shiba Toshikazu

  Division Of Molecular Chemistry Graduate School Of Engineering Hokkaido University

• SHIBA Toshikazu

  Graduate School of Engineering, Hokkaido University

• Noguchi Toshitada

  Biochemicals Division Yamasa Corporation

関連論文

• A Novel ATP Regeneration System Using Polyphosphate-AMP Phosphotransferase and Polyphosphate Kinase
• Enzymatic Synthesis of 2'-Deoxyguanosine with Nucleoside Deoxyribosyltransferase-II(Biochemistry & Molecular Biology)
• An Efficient Method for Production of Uridine 5'-Diphospho-N-Acetylglucosamine
• Novel Method for Enzymatic Synthesis of CMP-NeuAc(Biochemistry & Molecular Biology)
• Phosphopentomutase of Bacillus stearothermophilus TH6-2 : The Enzyme and Its Gene ppm
• Use of Escherichia coli Polyphosphate Kinase for Oligosaccharide Synthesis
• Enzymatic Synthesis of Cytidine 5'-Monophospho-N-acetylneuraminic Acid
• Molecular Cloning and Expression of the Nucleoside Deoxyribosyltransferase-II Gene from Lactobacillus helveticus

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