Rapid Discrimination of Mitochondrial DNA Type and Use of Results to Study Mitochondrial Inheritance in Pleurotus spp.

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概要

• 論文の詳細を見る

• We have reported a simple and rapid method to discriminate species in the genus Pleurotus by analysis of restriction-frament-length polymorphism of whole-cell DNA, and found that several restriction enzymes gave DNA bands useful in such discrimination, but other enzymes tested did not. In the present study, we report the reason why there were useful and useless enzymes; the effective enzymes digested rDNA into small fragments that did not interfere with the detection of DNA bands useful for discrimination. The origin of these discriminative DNA bands was found to be mitochondrial DNA when the banding profiles of whole-cell DNA, mitochondrial DNA, and nuclear DNA were compared. Consequently, our method could be used for rapid and simple identification of mitochondrial DNA type in the genus Pleurotus. The results were used to study mitochondrial inheritance, and we found that only the nucleus but not the mitochondria migrated during the mating of Pleurotus cornucopiae with P.citrinopileatus.

• 社団法人日本農芸化学会の論文
• 1998-04-23

著者

• Ando Akikazu

  Faculty Of Horticulture Chiba University

• Moriyama Y

  Fundamental Research Center Toto Ltd.

• Nagata Yoshiho

  Faculty Of Horticulture Chiba University

• Sonoe O

  National Food Research Institute

• SAGAWA Iwao

  Industrial Research Institute of Chiba Prefecture

• MORIYAMA Yuji

  Faculty of Horticulture, Chiba University

• Sagawa I

  Industrial Research Institute Of Chiba Prefecture

• Moriyama Y

  Nagoya City Univ. Nagoya Jpn

関連論文

• Degradation of PrP^ by Keratinolytic Protease from Nocardiopsis sp. TOA-1
• Purification and Some Properties of a Keratinolytic Enzyme from an Alkaliphilic Nocardiopsis sp. TOA-1(Microbiology & Fermentation Technology)
• Rapid Discrimination of Mitochondrial DNA Type and Use of Results to Study Mitochondrial Inheritance in Pleurotus spp.

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