A Very-high-density Lipoprotein with Clotting Ability from Hemolymph of Sand Crayfish, Ibacus ciliatus

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概要

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• A very-high-density lipoprotein (VHDL)with a density of 1, 27-1, 29g/ml was the most abundant lipoprotein in the hemolymph of the sand crayfish Ibacus ciliatus.The VHDL isolated by a density gradient ultracentrifugation consisted of 94% protein and 6% lipid reflecting its high density, and phospholipid was a perdominant lipid component.The VHDL had an apolipoprotein of molecular mass 195 kDa and its N-terminal amino acid sequence was identified as follows: LQPGLEYQYRYNGRVAA.This sequence was similar to those of clotting proteins from the spiny lobster Panulirus interruptus and the freshwater crayfish Pacifastacus leniusculus.Transglutaminase and Ca^<2+> also induced the VHDL to clot.Considering large amounts of VHDL in the hemolymph of sand crayfish, the VHDL not only functions as lipid carrier but plays an important role in the defense process of crustacea.

• 社団法人日本農芸化学会の論文
• 1998-03-23

著者

• Komatsu Masaharu

  Laboratory Of Food Chemistry

• ANDO Seiichi

  Lipoprotein Research Laboratory, Faculty of Fisheries, Kagoshima University

• Ando Seiichi

  Lipoprotein Research Laboratory Faculty Of Fisheries Kagoshima University

• Ando Seiichi

  Lipoprotein Research Laboratory Department Of Fisheries Science Kagoshima University

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