Cloning and Sequencing of the Genes for N-Acetylglucosamine Use That Construct Divergent Operons (nagE-nagAC) from Vibrio cholerae Non-O1
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概要
- 論文の詳細を見る
A 7.2-kb genomic DNA fragment containing N-acetylglucosamine-6-phosphate deacetylase gene (nagA) was cloned from the chitinase-producing bacterium Vibrio cholerae non-O1 strain 1148A (IFO 15429). Sequence analysis of the DNA fragment found three other complete open reading frames (ORFs) and the 5' end of an ORF. Amino acid sequences of two ORFs, ORF2 and ORF4, showed similarity with that of NagC, the repressor of nag operons and that of NagE, N-acetylglucosamine-specific transporter II^<Nag> of phosphoenolpyruvate transport system of Escherichia coli, respectively. In the presence of N-acetylglucosamine, nagA and ORF2 (nagC) were co-transcribed. ORF4 (nagC), which is upstream from nagAC but is expressed in the opposite direction was also transcriptionally induced in the presence of N-acetylglucosamine. These results indicated that nagE-nagAC existed as divergent operons in V. cholerae non-O1, Unlike E. coli, nagB and nagD were not in the operons.
- 社団法人日本農芸化学会の論文
- 1997-08-23
著者
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Wang Jingyu
工技院 大工研
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Wang Jingyu
Osaka National Research Institute Agency Of Industrial Science And Technology
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YAMANO Naoko
Osaka National Research Institute, Agency of Industrial Science and Technology
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OURA Noriyoshi
Osaka National Research Institute, Agency of Industrial Science and Technology
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FUJISHIMA Shizu
Osaka National Research Institute, Agency of Industrial Science and Technology
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Yamano Naoko
Osaka National Research Institute Agency Of Industrial Science And Technology
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Oura Noriyoshi
Osaka National Research Institute Agency Of Industrial Science And Technology
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Fujishima Shizu
Osaka National Research Institute Agency Of Industrial Science And Technology
関連論文
- 海洋低温細菌Alteromonas sp.の生産するN-アセチルグルコサミンデアセチラーゼ : 微生物
- Cloning and Sequencing of the Genes for N-Acetylglucosamine Use That Construct Divergent Operons (nagE-nagAC) from Vibrio cholerae Non-O1
- Purification and Characterization of N-Acetylglucosamine 6-Phosphate Deacetylase with Activity against N-Acetylglucosamine from Vibrio cholerae Non-O1
- Purification and Characterization of N-Acetylglucosamine-6-phosphate Deacetylase from a Psychrotrophic Marine Bacterium, Alteromonas Species
- 海洋細菌 Vibrio sp. によるキチン代謝