Purification and Characterization of N-Acetylglucosamine 6-Phosphate Deacetylase with Activity against N-Acetylglucosamine from Vibrio cholerae Non-O1

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概要

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• An enzyme that doacetylates N-acetylglucosamine to glucosamine from Vibrio cholerae non-O1 was purified to homogeneity by sequential procedures. The native enzyme had a molecular mass of 190,000 Da and was predicted to be composed of four identical subunits with molecular masses of 45,000 Da. The purified enzyme hydrolyzed N-acetylglucosamine, N-acetylglucosamine 6-phosphate, and N-acetylglucosamine 6-sulfate, but not chitin oligosaccharides, and N-acetylgalactosamine. The deacetylase activity was completely abolished by N-ethylmaleimide, p-chloromercuribenzoate, EDTA, and Cu^<2+>. On the other hand, the activity was activated by Co^<2+>. The amino-terminal amino acids of the purified enzyme were sequenced. Among the 22 N-terminal amino acid residues, 12 residues of Vibrio deacetylase were identical with that of Escherichia coli GlcNAc 6-phosphate deacetylase.

• 社団法人日本農芸化学会の論文
• 1996-08-23

著者

• YAMANO Naoko

  Osaka National Research Institute, Agency of Industrial Science and Technology

• FUJISHIMA Shizu

  Osaka National Research Institute, Agency of Industrial Science and Technology

• Yamano N

  Aist Kansai Osaka

• Yamamoto Noboru

  Green Biotechnology Research Group The Special Division For Human Life Technology National Insitute

• Yamano Naoko

  Osaka National Research Institute Agency Of Industrial Science And Technology

• Arita Michiko

  Research Institute Of Microbial Diseases Osaka University:faculty Of Health And Welfare Okayama Pref

• Fujishima Shizu

  Osaka National Research Institute Agency Of Industrial Science And Technology

• Fujishima Shizu

  Special Division Of Human Life Technology Aist Kansai National Institute Of Advanced Industrial Scie

• Matsushita Yasushi

  Department Of Orthopedic Surgery Cancer Institute Hospital

• Matusita Yasusi

  Department Of Orthopaedic Surgery Nagoya City University Medical School

• Matsushita Yasushi

  Osaka National Research Institute Agency Of Industrial Science And Technology

• Kamada Yoshitaka

  Osaka National Research Institute Agency Of Industrial Science And Technology

• Fujishima S

  Agency Of Industrial Sci. And Technol. Osaka Jpn

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