Variety of Intracellular Products among Recombinants Obtained by Interspecific Protoplast Fusion in Streptomycetes

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This paper describes with the multiformity of intracellular products among the recombinants obtained by interspecific protoplast fusion between Streptomyces griseus and S. griseoruber and between S. griseus and S. rochei. The products were extracted from the mycelium with methanol and analyzed by thin-layer chromatography using three detection methods. Detection with anisaldehyde-sulfuric acid reagent and Ehrlich's reagent showed that 29-37% of the recombinants produced new products different from those of the parental species, while about 50% of the recombinants did not produce any detectable substances. In a bioautogram against Bacillus subtilis, about 50% of the recombinants showed clear antibiotic activity in their methanol extracts, while the parental species did not. The distribution of the products (parental, mixed, new, or not expressed types) among the recombinants is discussed compared with that of taxonomic allelo-characters.
社団法人日本農芸化学会の論文
1997-04-23