Isolation and Characterization of a Plasmid from Lactobacillus helveticus CP53

スポンサーリンク

概要

• 論文の詳細を見る

• A plasmid, pCP53 (11.5 kilo base pairs long), was isolated from Lactobacillus helveticus CP53 and its restriction map was constructed. A chimeric plasmid, pCP53D (4.7 kb), which had a 2.0-kb HindIII fragment from pCP53 and a tetracycline resistance (Tc) gene from Streptococcus faecalis replicated and expressed tetracycline resistance in Lactobacillus casei CP680. Fifteen strains of L. helveticus were transformed with pCP53D by electroporation and selected for Tc resistance. Transformants were obtained from two out of 15 host strains, L. helveticus CP611 and CP791, with 5×10^3 and 50 transformants per μg of DNA, respectively.

• 社団法人日本農芸化学会の論文
• 1996-12-23

著者

• TAKANO Toshiaki

  R & D Center, The Calpis Food Industry Co., Ltd.

• YAMAMOTO Naoyuki

  R & D Center, The Calpis Food Industry Co., Ltd.

関連論文

• Decrease of Tissue Angiotensin I-Converting Enzyme Activity upon Feeding Sour Milk in Spontaneously Hypertensive Rats
• Isolation and Characterization of a Plasmid from Lactobacillus helveticus CP53
• Antihypertensive Effects of Different Kinds of Fermented Milk in Spontaneously Hypertensive Rats

スポンサーリンク