Detection of Protein A Produced by Staphylococcus aureus with a Fiber-optic-based Biosensor

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概要

• 論文の詳細を見る

• Staphylococcus aureus is a pathogen important in causing human infections and intoxication. A sensitive fiber-optic that produces evanescent waves was developed for the detection of protein A, a product secreted only by S. aureus. In the immunosensor, a 40-mV argon-ion laser that generated laser light at 488 nm was used together with plastic optical fiber and antibodies to protein A were physically adsorbed onto the fiber. The principle of the detection involved a sandwich immunoassay with fluorescein isothiocyanate conjugated with anti-(protein A) immunoglobulin G to produce signals of the antigen-antibody reaction. The detection limit was 1 ng of protein A per milliliter. The fiber-optic immunosensor could be used for rapid and specific detection of S. aureus in clinical specimens and foods.

• 社団法人日本農芸化学会の論文
• 1996-10-23

著者

• CHOU Chien

  School of Medical Technology, National Yang Ming Medical College

• Chou C

  National Yang‐ming Univ. Taipei Twn

• Chou Chien

  School Of Medical Technology National Yang Ming University

• Chou Chien

  School Of Medical Technology National Yang Ming College

• CHANG Ya

  School of Medical Technology, National Yang Ming University

• CHANG Tsung

  Food Industry Research and Development Institute

• KAO E-Fong

  School of Medical Technology, National Yang Ming University

• Chang Ya

  School Of Medical Technology National Yang Ming University

• Kao E-fong

  School Of Medical Technology National Yang Ming University

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