Determination of Glutathione and Related Aminothiols in Mouse Tissues by Gas Chromatography with Flame Photometric Detection
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概要
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A selective and sensitive method for the determination of glutathione and such related aminothiols as Cys, cysteinylglycine and γ-glutamylcysteine in mouse tissues by gas chromatography was developed. After fractionating a tissue sample into the free and protein-bound fractions, glutathione and aminothiols in each fraction were converted inito their N,S-isopropoxycarbonyl methyl ester derivatives and then measured by gas chromatography with flame photometric detection, using a DB-1 capillary column. This method enabled the redox status of glutathione and related aminothiols in mouse tissues to be investigated.
- 社団法人日本農芸化学会の論文
- 1996-04-23
著者
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Kataoka Hiroyuki
Faculty Of Pharmaceutical Sciences Okayama University
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Makita Masami
Faculty Of Pharmaceutical Sciences Okayama University
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TAKAGI Kiyomi
Faculty of Pharmaceutical Sciences, Okayama University
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Takagi Kiyomi
Faculty Of Pharmaceutical Sciences Okayama University
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