Formation of 1:1 Complex of the Cytokine Receptor Homologous Region of Granulocyte Colony-stimulating Factor Receptor with Ligand

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概要

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• The cytokine receptor homologous (CRH) region of the murine granulocyte colony-stimulating factor (G-CSF) receptor was secreted using a Escherichia coli maltose binding protein (MBP) fusion system. The CRH region was prepared from the periplasmic fraction by G-CSF affinity column chromatography and restriction protease factor Xa digestion, and was purified to homogeneity. The purified CRH region specifically bound G-CSF, with an apparent dissociation constant (Kd) of about 1. 5×10^<-9> M. A 1:1CRH・G-CSF complex was established by gel-filtration high pressure liquid chromatography (HPLC). However, a 2:1 stoichiometric complex was not established, as in the case of the growth hormone (GH)receptor [Recent Prog. Hormone Res. , 48, 233-275 (1993)].

• 社団法人日本農芸化学会の論文
• 1995-12-23

著者

• Ota Yoshimi

  Protein Engineering Research Institute

• Ota Y

  Hiroshima Univ. Hiroshima Jpn

• Hiraoka Osamu

  Protein Engineering Research Institute

• Anaguchi Hiroyuki

  Protein Engineering Research Institute

関連論文

• Deletion of D-Helix in Bovine Pancreatic Phospholipase A_2(Biological Chemistry)
• Convenient Desktop-scale Production of the Extracellular Domain of the Human Growth Hormone Receptor by an Insect-baculovirus Secretion System Using a Protein-free Culture
• Formation of 1:1 Complex of the Cytokine Receptor Homologous Region of Granulocyte Colony-stimulating Factor Receptor with Ligand

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