Convenient Desktop-scale Production of the Extracellular Domain of the Human Growth Hormone Receptor by an Insect-baculovirus Secretion System Using a Protein-free Culture

スポンサーリンク

概要

• 論文の詳細を見る

• Expression of a gene encoding the extracellular domain of the human growth hormone receptor (hGHR-ED) inserted into the genome of Autographa californica nuclear polyhedrosis virus was done using a desktop-scale spinner culture. Spodoptera frugiperda 9 (Sf9) cells infected with the recombinant virus secreted a protein with hGH-binding activity into the medium. Oxygen supplementation was required for high level secretion of the product. The highest cell production capability was estimated at more than 15 mg hGHR-ED/liter of culture. A protein-free medium supported the production similar to that obtained in traditional serum-containing media. This spinner culture system is simple to operate, and does not require expert knowledge of culture techniques.

• 1995-10-23

著者

• Ota Y

  Protein Engineering Research Institute

• Ota Yoshimi

  Protein Engineering Research Institute

• Minami M

  Protein Engineering Research Institute

• Asakura Akira

  Protein Engineering Research Institute

• Minami Mayumi

  Protein Engineering Research Institute

関連論文

• Deletion of D-Helix in Bovine Pancreatic Phospholipase A_2(Biological Chemistry)
• Convenient Desktop-scale Production of the Extracellular Domain of the Human Growth Hormone Receptor by an Insect-baculovirus Secretion System Using a Protein-free Culture
• Formation of 1:1 Complex of the Cytokine Receptor Homologous Region of Granulocyte Colony-stimulating Factor Receptor with Ligand

スポンサーリンク