Role of Glucose Dehydrogenase and Glucose Catabolism in Triggering of Spore Germination in Bacillus subtilis

概要

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Glucose dehydrogenase gene (gdh) mutants of Bacillus subtilis were construced using a derivative of the integration plasmid pJH101 to re-examine the previously reported result that glucose dehydrgenase was essential for triggering of spore germination. When spores were germinated in a complex medium containing glucose, the gdh-positive strain accumulated gluconate as the major metabolite, but no gluconate was found in the gdh mutant. Contrary to the previous study, our gdh mutant triggered spore germination normally on glucose in a minimal medium. Moreover a mutant having none of the activities for glucose dehydrogenase, glucose 6-phosphate isomerase, or glucose 6-phosphate dehydrogenase did not catabolized glucose, nevertheless the mutant triggered germination normally on glucose, thus it is concluded that neither of the major catabolic pathways for glucose is needed in germination triggering of B. subtilis spores.
社団法人日本農芸化学会の論文
1994-05-23