Inhibition of Soybean Lipoxygenase-1 by Sucrose Esters of Fatty Acids

概要

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The effect of sucrose esters of fatty acids with carbon numbers from 8 to 16 on soybean lipoxygenase-1 (L-1) was investigated. The sucrose esters inhibited L-1 and the inhibitory effect increased with an increase in the carbon number of the fatty acid up to 12, being constant above 12. Kinetic studies showed that two molecules of the sucrose ester bound to L-1 and inhibited it competitively. Based on the behavior of pK_i dependence on the carbon chain length of the sucrose esters, the dodecyl group appeared to be optimum to bind L-1. The results suggest that sucrose esters bind to the catalytic site of L-1 by hydrophobic interaction of the fatty acid and lead to the loss of activity. Sucrose esters, which are widely used as an emulsifier, can be an effective inhibitor of enzymatic lipid peroxidation in food processing.
社団法人日本農芸化学会の論文
1993-04-23