Transfer of Antisense Oligodeoxynucleotides Against Endothelin Receptors A and B Into Human Coronary Smooth Muscle Cells and Endothelial Cells by Apolipoprotein E Peptide : An in Vitro Study
スポンサーリンク
概要
- 論文の詳細を見る
Antisense oligodeoxynucleotides (AS-ODNs) are a new generation of therapeutic, agents for gene therapy. To develop a new approach in regulating the expression of endothelin (ET) receptor, N,N-dipalmitylglycyl-apolipoprotein E (129-169) peptide (dpGapoE), an efficient gene delivery system, was used to transfect phos-phorothioated AS-ODNs against nucleotides of human ET type A (ETA) receptors in human coronary smooth muscle cells (HCSMCs) and type B (ETB) receptors in human coronary endothelial cells (HCECs). After trans-fection, translocation to the nuclei and concentration in nuclear structures were observed in approximately 40% of HCSMCs and 60% of HCECs, respectively, at 48 h by fluorescence microscopy. Both the cellular ETA mRNA concentration in HCSMCs and ETB mRNA concentration in HCECs significantly declined. This approach may enable gene regulation in vivo and could be used to regulate vascular tone and constriction through ET receptors.
- 2002-10-20
著者
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SAKU Keijiro
Department of Cardiology, Fukuoka University Hospital
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Miura Shin-ichiro
Department of Cardiology, Faculty of medicine, Fukuoka University
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Saku Keijiro
Department Of Cardiology Fukuoka University Faculty Of Medicine
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Miura Shin-ichiro
Department Of Cardiology Fukuoka University Faculty Of Medicine
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Miura Shin-ichiro
Department Of Cardiology Fukuoka University School Of Medicine
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Saku Keijiro
Department Of Cardiology Fukuoka University School Of Medicine
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OKAMOTO Takehiro
Department of Internal Medicine, Fukuoka University, School of Medicine
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Okamoto Takehiro
Department Of Cardiology Fukuoka University School Of Medicine
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Via David
Department of Internal Medicine, Baylor College of Medicine
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Via David
Department Of Internal Medicine Baylor College Of Medicine
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Saku Keijiro
Department Of Cardiology Faculty Of Medicine Fukuoka University
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Miura Shin-ichiro
Department Of Cardiology Faculty Of Medicine Fukuoka University
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