7. トリプトファンの過剰蓄積がイネのインドール-3-酢酸含量に及ぼす影響

概要

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Effects of tryptophan over-production on the indole-3-acetic acid homeostasis in rice were investigated using the transformants expressing a feed-back insensitive form of anthranilate synthase a subunit gene (OASA1D). The over-expression of OASA1D transgene caused a drastic accumulation of Trp in two-week-old rice plant in a tissue-dependent manner. The Trp level in the youngest leaf of the transformant (HW5) reached ca 3000 nmol/gFW, while the increase in other leaves was more moderate. Stem and root also contained high amount of Trp. The level of IAA was increased by the OASA1D expression with the distribution pattern in the plant similar to that of Trp. The youngest leaf accumulated the highest amount of IAA, which was 20 times as much as that in untransformed rice plant (Nipponbare). No anomaly was observed in the morphology of the transformant. These results suggests that the IAA biosynthesis in OASA1D rice was activated as a result of drastic increase in the Trp level without affecting its growth.
植物化学調節学会の論文
2005-10-13