ヒト補体第1成分を用いた蛍光標識補体法の基礎的研究

概要

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The development of direct fluorescent complement technique has been done for demonstration of antigen-antibody reaction, because of the specificity of complement fixation reaction. 1 . For the specificity of this technique, human C1 was purified by the repeating of precipitation in low ionic strength and solubilization of euglobulin in high ionic strength at neutral pH. Purified C1 thus obtained showed high specific hemolytic activity. 2. Several conditions for the conjugation of FITC to the purified C1 were examined. Using the fluorescence labeled C1, specific fluorescence was observed in the following systems; sheep erythrocyterabbit anti sheep erythrocyte serum and MM2 (ascites form of mouse mammary tumor)-xenogeneic and syngeneic anti MM2 sera system. 3. When fluorescent C1 was separated into the three subcomponent fractions by Lepow's method, the strongest fluorescence was recovered from Clq fraction. 4. Specific fluorescence on the sensitized cells was not influenced by the addition of EDTA. However, it is known that C1q is capable of binding to antibody molecules in spite of the presence of EDTA. This phenomenon was discussed from this point, in connection with the existence of the majority of FITC on C1q moiety of C1 molecule.
日本アレルギー学会の論文
1970-02-28