リウマチ様因子の研究IgA-, IgG-rheumatoid factorの検出

概要

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The existence of IgG- and IgG-rheumatoid factors (RF) in addition to the IgM class was examined by antiglobulin augmentation technique. The IgG-aggregates sensitized tanned cell hemagglutination test was employed as described by Heller et al. After hemagglutination titers were recorded, the cells were washed three times with buffered saline at 4cC. After the third wash, the cells were resuspended in 0.5 ml of a dilution of the monospecific anti-globulin reagent. In all of the seven sera (five with rheumatoid arthritis and two with chronic hepatitis), IgA-RF was detected. IgG-RF was proven to be present in four cases. A rheumatoid serum sample used here had titer of 1 : 5, 120 to equine IgG-aggregates. This original titer was augmented to 1 : 40, 960, 1 : 10, 240 and 1 : 163, 840 by anti-IgM, anti-IgA and anti-IgG, respectively. After the treatment of 2-ME, the RF-activity in original titration was completely inactivated and no enhancement was noted with anti-IgM or anti-IgA. In contrast, the IgG augmentation was still observed. In addition, the enhancement with anti-IgG was noted in IgG was noted in IgG-fraction (DEAE cellulose chromatography) which showed no RF-activity in original titration. These results favour the assumption that the IgG-RF, in some of the sera with rheumatoid arthritis, would be univalent.
日本アレルギー学会の論文
1969-02-28