Stem Cell Quality Following Irradiation: Comparison of ^<125>IUdR Uptake in the Spleen and Number and Size of Splenic Colonies in Bone Marrow Transfused, Fatally Irradiated Mice1

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In order to study radiation induced residual injury in hemopoietic stem cells the number and size of spleen colonies were compared to the incorporation of ^<125>I-labeled iododeoxyur-idine (^<125>IUdR) in the spleen after transfusion of irradiated bone marrow cells in the lethally irradiated mouse. Bone marrow cells from non-irradiated or from mice that had been given 3,000 rad in daily 50 rad increments 5 days per week were used 3 months after termination of irradiation. Marrow cells from donor mice given 50 rad immediately prior to the experi-ment were comparably used to study acute radiation effect. The splenic proliferation factor (PF) (ratio of 5th to 3rd ^<125>IUdR incorporation) of 7.3 ± 0.2 for the 3,000 rad irradiated bone marrow is 44.2% of the control value (16.5 ± 0.9). Spleen colonies produced by stem cells from 3,000 rad mice are 40-50% of the size of colonies produced by nonirradiated stem cells.
日本放射線影響学会の論文