Cysteamine Rapidly Decreases Mitotic Cells in Random Culture of Hela S-3 Cells

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Mitotic cells disappeared nearly completely within 60 mm after the addition of 30 mM cysteamine in HeLa S-3 cell culture, while the control culture contained mitotic cells with the frequency of 3.4%. Inhibition of mitosis became gradually irreversible when the treatment was extended over 60 mm, but cytolysis occurred much more slowly. Ethanolamine exhibited a similar but much less significant effect. In addition to the well-known radioprotective action, aminothiol derivatives such as cysteamine (2-mercaptoethylamine) and WR-2721 (S-2[3-aminopropylamino] ethylphosphorothioic acid) are known to have an oncostatic action. 1, 2) Chemical modification of either amino or thiol group of cysteamine results in marked reduction of the oncostatic activity of the compound1) as well as its radioprotective activity. 1, 3) The length of the carbon chain between the two groups also is implicated in both activities of the compound. It has thus been assumed that '2-point attachment' is requisite for the actions of cysteamine, although it is unclear what component of the cells is the principal target of its binding.
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