STUDIES ON THE CHROMOSOME BY SCANNING ELECTRON MICROSCOPE : A NEW TRIAL WITH LONG TIME GIEMSA STAINING
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概要
- 論文の詳細を見る
光学顕微鏡(以下LM)下で良好なBandが観察される染色体は厚みが薄すぎて走査型電子顕微鏡(以下SEM)での観察には望ましくないと言われてきたが, 我々はギムザ染色液の染色体の厚みを増す効果に注目し, 従来試みられたことのない長時間ギムザ染色法を用いて標本を作成し, SEMにて観察し以下の結果を得た. 1) トリプシン処理時間を一定にしギムザ染色を30分間から24時間まで行つたところ時間経過に比例して染色体は厚みを増しFibrous structureも明瞭になつてくる. 2) 長時間ギムザ染色を行つた染色体は厚みは十分であるが時間経過に比例して Bandは消失していく傾向が認められた. 3) トリプシン処理を行わない長時間ギムザ染色法では Chromatin fiberが密にたたまれている染色体が観察されたことからトリプシン処理は fiberのからみあいを looseにすることが考えられた. 以上のような形態学的特徴から本法は Satellite associationや染色体構造異常の研究に有用であると思われる.
- 社団法人日本産科婦人科学会の論文
- 1988-07-01
著者
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YAJIMA AKIRA
Department of Obstetrics and Gynecology, Tohoku University School of Medicine
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TAKABAYASHI Toshifumi
Department of Obstetrics and Gynecology, Tohoku University School of Medicine
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Yajima Akira
Department Of Obstetrics And Gynecology Tohoku University School Of Medicine
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Takabayashi Toshifumi
Department Of Obstetrics And Gynecology Tohoku University School Of Medicine
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SASAKI Hiroyuki
Department of Physics, Graduate School of Science, Tohoku University
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OZAWA Nobuyoshi
Department of Obstetrics and Gynecology, Tohoku University School of Medicine
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SASAMOTO Kiyo
Department of Obstetrics and Gynecology, Tohoku University School of Medicine
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SHINTAKU Yuko
Department of Obstetrics and Gynecology, Tohoku University School of Medicine
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Shintaku Yuko
Department Of Obstetrics And Gynecology Tohoku University School Of Medicine
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Sasamoto Kiyo
Department Of Obstetrics And Gynecology Tohoku University School Of Medicine
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Ozawa Nobuyoshi
Department Of Obstetrics And Gynecology Tohoku University School Of Medicine
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Sasaki Hiroyuki
Department Of Obstetrics And Gynecology Tohoku University School Of Medicine
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Sasaki Hiroyuki
Department Of Information Systems Science Faculty Of Engineering Soka University
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