Comparative Studies of the Hanabusaya asiatica and its Allied Groups by RAPD Analysis(Molecular Systematics)

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概要

• 論文の詳細を見る

• The phylogenetic relationships between Hanabusaya asiatica and its allied groups were assessed by Randomly Amplified Polymorphic DNA (RAPD) analysis of genomic DNA, and their taxonomic status was reevaluated at the molecular level. The analyzed plants consisted of 36 populations of eight genera and 27 taxa. Forty-two out of 101 primers (10-mer) screened were able to amplify DNA. Only 19 primers, however, actually succeeded in DNA amplification, resulting in 523 randomly amplified DNA fragments. The analyzed taxa showed very high polymorphism, allowing each individual taxon to be identified based on RAPD analysis. All eight genuses were differentiated from each other at the 0.86 level of similarity index value. In particular, Hanabusaya asiatica was distinguished from its closely related genus. Intraspecific and interspecific relationships were quite close, at levels ranging from 0.77 to 0.99. Our RAPD analysis supports the previous data based on morphological and palynological studies.

• 種生物学会の論文

著者

• Lee Woo

  Department of Mechanical Engineering, Seoul National University

• Kim Nam

  Division of Cardiology, Chonnam National University Hospital

• Kim N

  Seoul National Univ. Seoul Kor

• Lim Hak

  Division Of Applied Plant Sciences Kangwon National University

• Lee Woo

  Department Of Biological Scienceand Basic Science Research Institute Sungkyunkwan University

• YOO KI

  Department of Biology, Kangwon National University

• Yoo Ki

  Department Of Biology Kangwon National University

• Lee Woo

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences Univers

• KIM NAM

  Division of Applied Plant Sciences, Kangwon National University

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