Microspore Mutagenesis and In Vitro Selection for Resistance to Soft Rot Disease in Chinese Cabbage (Brassica campestris L. ssp. pekinensis)
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概要
- 論文の詳細を見る
Plants resistant to soft rot disease were produced by in vitro-mutagenesis and -selection using a microspore culture system in Chinese cabbage. The effect of ultra-violet (UV) irradiation on microspore embryogenesis was investigated. Embryo production decreased with increasing duration of UV-irradiation and the LD_<50> was estimated to correspond to an exposure of 12 s. Culture filtrate of soft rot bacteria caused a reduction of embryo formation from microspores and plant regeneration from microspore-derived embryos, suggesting that the culture filtrate is phytotoxic. After 6,657 embryos derived from UV-irradiated microspores were selected on a medium containing a soft rot culture filtrate, 250 plantlets were regenerated. When their progenies were assessed for resistance to soft rot disease, three lines showing a markedly increased resistance to the disease were obtained. The resistant plants are considered to be mutants produced by in vitro-mutagenesis.
- 日本育種学会の論文
著者
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TAKAHATA Yoshihito
Faculty of Agriculture, Iwate University
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Takahata Yoshihito
Faculty Of Agnculture Lwate Untversity
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Feng-Ian Zhang
Faculty of Agnculture lwate Untversity
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Feng-lan Zhang
Faculty of Agnculture lwate Untversity
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