6-2) Release of Liver Microsomal β-Glucuronidase From Hepatocytes in vitro and in vivo by Organophosphates and Hepatotoxic Agents

概要

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Liver microsomal β-glucuronidase is stabilized within microsomal vesicles by complexation with the accessory protein, named egasyn. In this study, we showed that egasyn is identical to one of the carboxylesterase isozymes and organophosphorus and carbamate insecticides, acetanilide which is a specific substrate of egasyn and halothane caused a rapid dissociation of the egasyn-microsomal β-glucuronidase complex when administered in vivo or when added in vitro to isolated hepatocytes. The dissociation was relatively specific to organophosphates, carbamates, but not pyrethroids. Dissociation of the egasyn-β-glucuronidase complex in vivo by organophosphates was followed by massive and rapid secretion of microsomal β-glucuronidase into plasma. From these results, we concluded that release of liver microsomal β-glucuronidase is the most rapid and sensitive marker to organophosphorus or carbamate insecticide-induced intoxication.
日本トキシコロジー学会の論文
1991-02-28