全自動放射拡散螢光免疫測定法による血清中ジゴキシン濃度測定の検討

概要

論文の詳細を見る
The radial partition fluorometric enzyme immunoassay (RPIA) was studied for determination of digoxin concentration in the serum. In 3 levels of digoxin added sample, the coefficients of variation (CV) values of within-run from 5 replicate assays were 1.58-12.2% in digoxin spiked samples at 3 levels. The mean recovery of digoxin by RPIA was 94.7-108.8% from 5 kinds of concentration known samples. The CV values obtained from a low level (0.5ng/ml) of digoxin were greater than those from middle (1.0ng/ml) and high (2.0ng/ml) levels of the drug. The constituents in the blood such as proteins, triglycerides and hemoglobin had little influence on this analysis. There was a significant correlation between digoxin concentrations of 50 samples determined by both RPIA and fluorescence polarization immunoassay (FPIA), although the values determined by RPIA tended to be slightly higher than those by FPIA. RPIA seemed to be a reliable and useful technique for rapid determination of digoxin concentrations since the method is able to precisely determine digoxin in the serum even in the presence of hemolysis or chylomicronemia and moreover requires no pretreatment of deproteinization.
日本医療薬学会の論文
1988-12-20