PURIFICATION AND CRYSTALLIZATION OF CLOSTRIDIUM BOTULINUM TYPE C TOXIN
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概要
- 論文の詳細を見る
A procedure for purification and crystallization of Clostridium botulinum type C toxin is described. The toxin was precipitated from the culture by addition of zinc chloride and eluted by dibasic sodium phosphate solution from the precipitate. The toxin was precipitated with ammonium sulfate to 34% saturation at 4℃ and the toxin solution dissolved in acetate-phosphate buffer was chromatographed by the connected columns of Sephadex G-75-DEAE cellulose-QAE Sephadex A-50 at pH 7.0. The eluate had a specific toxicity of 3.8×10^7 MLD/1.0 E_<277.5mμ>. The toxin was crystallized from the solution dialysed against the saturated ammonium sulfate solution by ultrafiltration. The crystalline toxin had a specific toxicity of 3.0×10^7 MLD/1.0 E_<277.5mμ> and a faint hemagglutination activity. The crystalline toxin contained at least two components in acrylamide gel electrophoresis and the agar gel immunodiffusion test, respectively.
- 北海道大学の論文
著者
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Syuto Bunei
北海道大学 獣
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Syuto Bunei
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University
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Kubo Shuichiro
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University
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