土壌有機燐の作物による利用に関する研究(第4報) : 土壌有機燐に分別について

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Fractionation of organic phosphorus in soil and the properties of the easily dephosphorylable fraction were investigated this time. The results may be summarized as follows : 1. The BOWER'S method, which was considered to fractionate organic phosphorus, extracted from soil by 2% NaOH, into two types, phytin and nucleic acid, was proved by the authors not to be successful in some soils of this experiment (Table 1). 2. "Nucleotide" precipitation, isolated by the method of DYER using 4% NH_4OH for extraction, was more resistant to the dephosphorylation by rice bran enzyme than the filtrate from the above precipitation (Table 2 and Fig. 2). 3. Organic phosphorus in the Ca filtrate, extracting soil with 2 volumes of 2% NaOH at 60°, was dephosphorylated by the action of alkali phosphatase and easily oxidised by alkaline hypobromite, thus the phosphorus in this filtrate was presumed to be of a nucleic acid-like form (Table 3 and Fig. 3〜4).
社団法人日本土壌肥料学会の論文
1955-11-05