各種抽出液によって抽出される可給態窒素の土壌中の存在形態

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In order to identify the form of available nitrogen in soils, we examined the characteristics of organic nitrogen and quantitative relation between organic nitrogen and metallic ion on several soils extracted by chemical solutions such as dilute sulfuric acid. The results are summarized as follows : 1) According to the chromatograms of size-exclusion and ion-exchange HPLC, the organic nitrogen extracted by dilute sulfuric acid, phosphate buffer, ammonium acetate and distilled water are considered to be almost homogeneous protein-like substances. 2) Ferrum and aluminum were estimated to be combined with protein-like substances but the contribution is different by soil condition. In paddy fields, the contribution of ferrum in combination with protein-like substances is relatively high in terms of the amount of organic matter in the soil, while in upland soil, aluminum contribution is very high and it seemed that ferrum was combined with sparingly resolvable protein-like substances. But soils used in this experiment were classified as light-colored Andosol containing a large amount of active aluminum, therefore the contribution of ferrum and aluminum in upland soil except Andosol is the subject for a future study. 3) In the soils fertilized by a heavy dose of manure, water-soluble organic nitrogen tends to increase. This suggests that the capacity of soils for protein-like substances is limited and that organic matter applied in excess of capacity causes protein-like substances to be water-soluble and flow into the environment. 4) The estimation of available nitrogen by the chemical extraction method sometimes includes a large error compared with results using an incubation method. We inferred a fraction that mineralized easily because of a very weak combination with organo-minerals caused an error by salting out through the extraction. Additional studies on the properties of organic nitrogen extracted from soils fertilized by heavy doses of manure are required.
社団法人日本土壌肥料学会の論文
2000-12-05

各種抽出液によって抽出される可給態窒素の土壌中の存在形態

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